Journal: eLife

Article Title: Superoxide dismutases maintain niche homeostasis in stem cell populations

doi: 10.7554/eLife.96446

Figure Lengend Snippet: ( A–F ) Distribution of Tj + CySCs ( A’-B’ ) and Vasa + GSCs ( C’’-D’’ ) in control ( nos-Gal4/+ ) compared to that of nos-Gal4/SOD1i was imaged ( A–D ) and quantified ( E–F ). ( G–M ) Changes in the number of Tj + ( G’’ , H’’ and I’’ ) and Vasa + cells ( G’ , H’ , and I’ ) when Sod2 was depleted either in CySCs (using Tj-Gal4 driver) ( I , J and K ) or GSCs (using Nos-Gal4 driver) ( H , L , and M ). Data denotes mean ± s.e.m, n=10. ( N ) Immunoblot using Vasa-specific antibody showing changes in overall Vasa levels when Sod1 was knockdown. β-tubulin was used as loading control. ( O–P ) Assessment of cell proliferation in Tj>Sod1 i ( O ) and Nos >Sod1 i ( P ) using UAS-fluorescent ubiquitination-based cell cycle indicator (FUCCI) reporter line, n=10. ( Q ) Quantitative RT-PCR reflecting the fold change difference of CyclinD expression in Tj>SOD1 i testes compared to control. Data is depicted as mean ± s.e.m, n=3. ( R–S ) Representative images showing variations in Zfh1 + cells present in different phases of cell cycle among control (R: I-VII) and experimental flies (S: I-VII). (R:V & S:V) shows co-localisation of Zfh1 with S-phase cell. Scale bar: 10 µm, ns: non-significant. Data points denote mean ± s.e.m, n=10. ( T–U ) Control ( T ) and Tj-Gal4 driven Sod1RNAi ( U ) testes stained with pH3, a mitotic marker denoting the cell proliferation. For all graphs *** p (unpaired t-test)<0.0001, **p (unpaired t-test)<0.001 . Scale bar: 10 µm.Data denotes mean ± s.e.m, n=10. Figure 2—figure supplement 1—source data 1. Individual original raw unlabelled blots to . Figure 2—figure supplement 1—source data 2. Labelled original blots corresponding to .

Article Snippet: The following primary antibodies were used in the experiments - anti-FasIII (7G10) (1:120, DSHB (Developmental Studies Hybridoma Bank)), anti-Eya (1:50, DSHB), anti-DEcad (DCAD2) (1:50, DSHB), anti-α-Spectrin (3A9) (1:20, DSHB), anti-Ptc (1:50, DSHB), anti-Ci (1:50, DSHB), anti-Dlg (1:50), anti-β tubulin (1:300, DSHB), anti-pERK (1:100, Cell Signalling (4370)), ATP5A (1:700, Abcam (ab14748)), anti-Tj (1:5000), anti-Vasa (1:4000), anti-Zfh1 (1:2000).

Techniques: Control, Western Blot, Knockdown, Ubiquitin Proteomics, Quantitative RT-PCR, Expressing, Staining, Marker

Journal: bioRxiv

Article Title: Activation of TPC2 amplifies lysosome-mitochondria calcium transfer to regulate energetic stress responses

doi: 10.64898/2026.03.03.709381

Figure Lengend Snippet: ( A ) Representative images of Nestin+, SOX2+ neuronal progenitor cells and mature neurons (identified using βIII-Tubulin and MAP2) after 30 days of differentiation. Nuclei stained using Hoechst 33342. ( B-D ) Percentage cell death in mature human iPSC-derived neurons in response to ( B ) 6 hrs, ( C ) 8 hrs or ( D ) 10 hrs of oxygen-glucose deprivation (OGD) alone (solid bars) or OGD with 24 hrs of reperfusion (dashed bars) which, as expected, induces substantially more cell death. ( E ) Percentage cell death in human iPSC-derived neurons in response to 6 hrs of OGD and 24 hrs of reperfusion in the presence of increasing doses of Ned-19 or Ned-K. ( F ) As E but 8 hrs of OGD. ( G ) Percentage cell death in mouse N2A cells in response to 4 hrs of OGD and 24 hrs of reperfusion in the presence of increasing doses of Ned-19 or Ned-K. ( H ) As in G but 6 hrs of OGD. Nx = normoxia, Veh. = vehicle treatment in the absence (grey bars) or presence (colored bars) of OGD. B-D analyzed using a two-way ANOVA and E-H analysed using a repeated measures two-way ANOVA with Tukey post-hoc analysis. ***p ≤ 0.001, ††p ≤ 0.01 vs Vehicle for Ned-19 treated group, #p ≤ 0.05 vs Vehicle for Ned-K treated group. Panels B-C, n=3-6/group. Panels E=H, n=5/group.

Article Snippet: Characterization of NPCs and 30-day differentiated KOLF hiPSCs was done by immunostaining using the NPC markers Nestin (mouse anti-human, 1:250; [R&D systems; #656801]), SOX2 (goat anti-human SOX2, 1:100; [R&D systems #AF2018-SP]), the mature neuronal marker microtubule-associated protein 2 (MAP2) (rabbit anti-human MAP2, 1:200; [Genetex GTX133109]), and β-III tubulin (mouse anti-human β-III tubulin, 1:500; [DSHB #E7]).

Techniques: Staining, Derivative Assay